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Test Code
This test is a lab developed test. For further information, see the Compliance Remarks section below.
SynonymsCotinine, Nicotine
Container TypeRed top tube (plain)
Supply Item Number1372
Specimen TypeSerum
Preferred Volume1 mL
Emergency Minimum Volume0.5 mL
Specimen ProcessingSeparate serum from cells within 2 hours and transfer to a standard PAML aliquot tube.
Store and TransportRefrigerated
StabilityRoom Temp 1 week  Refrigerated 1 week  Frozen (-20 °C) 4 weeks  
Unacceptable ConditionWhole blood or grossly hemolyzed specimens
Alternate SpecimensSerum or plasma separator tubes
LimitationsIf testing is to be delayed by more than 1 week, specimens should be stored frozen for up to 1 month prior to being tested. Avoid repeated freezing and thawing of the specimen. NOTE: Samples sitting in gel SST for prolonged times may reduce drug concentrations significantly.
Reference LaboratoryPAML
Reference Lab Test CodeLCA code 910974
CPT Codes80307
Interface Order Code80302.Z1
Billing CodeCOTQ
Test ScheduleTue, Fri
Turnaround Time1-5 days
MethodEnzyme Multiplied Immunoassay
Reference Ranges
CotinineDaily Smokers> 100ng/mL
Light Smokers15-100
Passive or Non Smokers< 15
Clinical SignificanceHeavy secondhand smoke exposure has shown results up to 25 ng/mL in serum. Thus, an overlap may occur between cotinine concentrations of a non-smoker who experiences heavy secondhand smoke exposure and light or occasional smokers.
Compliance RemarksThis test was developed and its performance characteristics determined by PAML/PSHMC Division of Laboratory Medicine. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test. However, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. PAML/PSHMC is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.

Interface Codes
typeorder codetest descriptionresult coderesult descriptionunitsdecimal placesloinc code
o80302.Z1Cotinine, Serum
r80302.Z1Cotinine, Serum80302.Z1Cotinine, Serumng/mL010365-5