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Test Code
This test is a lab developed test. For further information, see the Compliance Remarks section below.
Synonyms3-Hydroxycotinine; Cotinine; Nicotine; Nicotine and Cotinine
Container TypeRed top tube (plain)
Supply Item Number1372
Specimen TypeSerum
Preferred Volume4 mL
Emergency Minimum Volume1 mL
Specimen ProcessingSeparate serum or plasma from cells ASAP or within 2 hours of collection and transfer to a standard PAML aliquot tube.
Store and TransportRefrigerated
StabilityRoom Temp 1 week  Refrigerated 2 weeks  Frozen (-20 °C) 1 month  
Unacceptable ConditionPlasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to more than 3 freeze/thaw cycles
Alternate SpecimensGreen (sodium heparin), lavender (EDTA), pink (K2EDTA), or gold, brick, SST, or corvac (serum separator tube)
Reference LaboratoryPAML
CPT Codes80323, (HCPCS G0480)
Interface Order Code83887.Z10
Billing CodeNICMSP
Test ScheduleTue, Thu
Turnaround Time1-5 days
MethodTandem Mass Spectrometry
Reference Ranges
NicotineNon-smoker< 2ng/mL
CotinineNon-smoker< 5ng/mL
3-OH-CotinineNon-smoker< 10ng/mL
> 5 ng/mL Nicotine indicates use of tobacco products and or nicotine replacement therapy.
> 100 ng/mL Cotinine indicates use of tobacco products and or nicotine replacement therapy. Cotinine is the major metabolite of nicotine.
> 100 ng/mL of 3-OH Cotinine indicates use of tobacco products and/or nicotine replacement therapy.
Compliance RemarksThis test was developed and its performance characteristics determined by PAML. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test. However, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. PAML is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.
NotesUseful for monitoring tobacco use, as well as, monitoring patients on nicotine-replacement therapy for concurrent use of tobacco products.

Interface Codes
typeorder codetest descriptionresult coderesult descriptionunitsdecimal placesloinc code
o83887.Z10Nicotine and Metab Serum-Plasma
r83887.Z10Nicotine and Metab Serum-Plasma83887.Z7Nicotine Serum-Plasmang/mL03853-9
r83887.Z10Nicotine and Metab Serum-Plasma83887.Z8Cotinine Serum-Plasmang/mL010365-5
r83887.Z10Nicotine and Metab Serum-Plasma83887.Z93-OH-Cotinine Serum-Plasmang/mL035140-3