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LYMPHOCYTE SUBSETS

Test Code
TBNK
 
SynonymsNatural Killer Cells
SPECIMEN REQUIREMENTS
Container TypeYellow top tube (ACD type A or B) and lavender top tube (EDTA)
Supply Item Numberpicture1055 and picture1495
Specimen TypeWhole blood
Preferred Volume7 mL ACD and 5 mL EDTA
Minimum Volume5 mL ACD and 2.5 mL EDTA
Store and TransportAmbient (room temperature)
Required Patient InfoIn accordance with CDC guidelines please provide the following patient infromation: WBC count and percent lymphocytes on the day of collection if sample will arrive after 24 hours.
StabilityRoom Temp 3 days  
Unacceptable ConditionEDTA tube is only for WBC and % lymph counts. Cannot be sent by itself for antibody testing. Samples cannot be refrigerated.
LimitationsSamples must be processed within 3 days of collection
CPT Codes86360, 86355, 86359, 86357
Billing CodeTBNK
Test ScheduleDaily
Turnaround Time2 days
MethodFlow Cytometry
Test IncludesSource, WBC K/uL; Lymphocytes, %; Lymph Abs, K/uL; CD3, %; CD3 Abs, /uL; CD4, %; CD4 Abs, /uL; CD8, %; CD8 Abs, /uL; CD19, %; CD19 Abs, /uL; CD56+16, %; CD56+16 Abs; CD4/CD8 Ratio; Note; Note
Reference Ranges
Source                  
WBC                                          K/uL                  
                  0 days       9.0-30.0                  
                  1-7 days     5.0-21.0                  
                  7-30 days    5.0-19.5                  
                  1-12 mo      6.0-17.5                  
                  1-2 yrs      5.0-15.5                  
                  2-4 yrs      6.0-15.5                  
                  4-6 yrs      5.0-13.5                  
                  6-10 yrs     4.5-13.5                  
                  10-14 yrs    5.0-11.0                  
                  14-18 yrs    4.5-11.0                  
                  18 yrs+      3.8-11.0                   
Lymphocytes       Newborn      9.0-46.0      %                           
                  1-3 days     16.0-46.0                                                        
                  4-7 days     26.0-56.0                                                        
                  8-14 days    33.0-63.0                                                        
                  15-60 days   41.0-71.0                                                        
                  2-11 mo      46.0-76.0                                                        
                  1-4 yrs      35.0-76.0                                                        
                  5-12 yrs     23.0-57.0                                                        
                  13-18 yrs    20.0-50.0                                                        
                  19+ yrs      15.0-48.0                                                        
Lymphs, Abs       Newborn      2.00-11.00    K/uL                  
                  1-3 days     2.00-11.50                  
                  4-7 days     2.00-17.00                  
                  8-14 days    2.00-17.00                  
                  15-60 days   2.50-16.50                  
                  2-11 mo      4.00-13.50                  
                  1-4 yrs      2.00-10.50                  
                  5-12 yrs     1.50-7.00                  
                  13-18 yrs    1.20-5.20                  
                  19+ yrs      1.00-3.90                  
CD3               0-3 yrs      55.0-82.0     %                           
                  4+ yrs       53.0-91.0                                                        
CD3 Absolute      0-5 mo       3500-5000     /uL                         
                  6-11 mo      3400-4600                                                        
                  12-17 mo     3200-3900                                                        
                  18-29 mo     2800-3500                                                        
                  30 mo-35 mo  1900-3100                                                        
                  3 yrs        1000-3900                                                        
                  4+ yrs       560-3000                                                         
CD4               0-5 mo       50.0-57.0     %                           
                  6-11 mo      49.0-55.0                                                        
                  12-17 mo     46.0-51.0                                                        
                  18-23 mo     42.0-48.0                                                        
                  24-29 mo     38.0-46.0                                                        
                  30-35 mo     33.0-44.0                                                        
                  3 yrs        27.0-57.0                                                        
                  4+ yrs       30.0-65.0                                                        
CD4 Absolute      0-5 mo       2800-3900     /uL                         
                  6-11 mo      2600-3500                                                        
                  12-17 mo     2300-2900                                                        
                  18-23 mo     1900-2500                                                        
                  24-29 mo     1500-2200                                                        
                  30-35 mo     1200-2000                                                        
                  3 yrs        560-2700                                                         
                  4+ yrs       490-1400                                                         
CD8               0-2 yrs      8.0-31.0      %                           
                  3 yrs        14.0-34.0                                                        
                  4+ yrs       12.0-38.0                                                        
CD8 Absolute      0-2 yrs      350-2500                                                                                  
                  4+ yrs       225-900                                                          
CD19              0-2 yrs      11.0-45.0     %                           
                  3 yrs        9.0-29.0                                                         
                  4+ yrs       3.0-21.0                                                         
CD19 Absolute     0-2 yrs      430-3300      /uL                         
                  3 yrs        200-1300                                                         
                  4+ yrs       80-450                                                           
CD56+16                                      %
CD56+16 Absolute                  /uL
CD4/CD8 Ratio     0-2 yrs      1.17-6.22     to 1.0                      
                  3 yrs        0.98-3.24                                                        
                  4+ yrs       1.00-3.00                                                                                                                     
Note                                                                                            
Note

Interface Codes
typeorder codetest descriptionresult coderesult descriptionunitsdecimal placesloinc code
o86999.Z296Lymphocyte Subsets
r86999.Z296Lymphocyte Subsets86999.Z295CD56+16, Absolute/uL020402-4
r86999.Z296Lymphocyte Subsets88180.Z10Lymphocytes, AbsoluteK/uL 30364-4
r86999.Z296Lymphocyte Subsets88180.Z14CD3% 8124-0
r86999.Z296Lymphocyte Subsets88180.Z16CD3, Absolute/uL 8122-4
r86999.Z296Lymphocyte Subsets88180.Z17CD4% 8123-2
r86999.Z296Lymphocyte Subsets88180.Z19CD4, Absolute/uL 8127-3
r86999.Z296Lymphocyte Subsets88180.Z22CD8% 8101-8
r86999.Z296Lymphocyte Subsets88180.Z24CD8, Absolute/uL 14135-8
r86999.Z296Lymphocyte Subsets88180.Z29CD19% 8117-4
r86999.Z296Lymphocyte Subsets88180.Z31CD19, Absolute/uL 8116-6
r86999.Z296Lymphocyte Subsets88180.Z35CD56+16% 
r86999.Z296Lymphocyte Subsets88180.Z43CD4/CD8 Ratioto 1.0 54218-3
r86999.Z296Lymphocyte Subsets88180.Z6Source  31208-2
r86999.Z296Lymphocyte Subsets88180.Z7WBCK/uL 6690-2
r86999.Z296Lymphocyte Subsets88180.Z92Lymphocytes% 30365-1
r86999.Z296Lymphocyte Subsets88180.Z93Note  55230-7
r86999.Z296Lymphocyte Subsets88180.Z94Note  55230-7

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