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Test Code
This test is a lab developed test. For further information, see the Compliance Remarks section below.
SynonymsEstrogens, fractionated; Estrone (E1) and Estradiols (E2); Total Estrogens; ultrasensitive
Container TypeRed top tube (plain)
Supply Item Numberpicture1373
Specimen TypeSerum
Preferred Volume0.5 mL
Minimum Volume0.3 mL
Specimen ProcessingSeparate serum from cells, transfer to 2 standard PAML aliquot tubes. If using SST tube, serum must be separated from gel within 24 hours of draw.
Store and TransportRefrigerated
StabilityRoom Temp 4 days  Refrigerated 1 week  Frozen (-20 °C) 1 month  Frozen (-70 °C) 1 month  
Alternate SpecimensSerum separator tube (gold, brick, SST, or corvac); lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin)
CPT Codes82679 x 1, 82670 x 1
Interface Order Code82671.Z7
Billing CodeESTF
Test ScheduleTue, Thu, Sat
Turnaround Time2-4 days
MethodTandem Mass Spectrometry
Reference Ranges
Estradiol by TMSMaleTanner Stage ILT 8pg/mL
Tanner Stage IILT 10
Tanner Stage III1-35
Tanner Stage IV AND V3-35
7-9 yrsLT 7
10-12 yrsLT 11
13-15 yrs1-36
16-17 yrs3-34
18+ yrs10-42
FemaleTanner Stage ILT 56
Tanner Stage II2-133
Tanner Stage III12-277
Tanner Stage IV and V2-259
7-9 yrsLT 36
10-12 yrs1-87
13-15 yrs9-249
16-17 yrs2-266
18+ yrs
Early Follicular30-100
Late Follicular100-400
Estrone by LCMSMSMaleTanner Stage ILT 7pg/mL
Tanner Stage IILT 11
Tanner Stage III1-31
Tanner Stage IV AND V2-30
7-9 yrsLT 7
10-12 yrsLT 11
13-15 yrs1-30
16-17 yrs1-32
18+ yrs9-36
FemaleTanner Stage ILT 27
Tanner Stage II1-39
Tanner Stage III8-117
Tanner Stage IV and V4-109
7-9 yrsLT 20
10-12 yrs1-40
13-15 yrs8-105
16-17 yrs4-133
18+ yrs
Early FollicularLT 150
Late Follicular100-250
Estrogens Total Calculation (Estrone + Estradiol)MaleTanner Stage I1-11pg/mL
Tanner Stage II1-19
Tanner Stage III3-61
Tanner Stage IV AND V4-62
7-9 yrsLT 10
10-12 yrs1-19
13-15 yrs3-62
16-17 yrs4-64
18+ yrs19-69
FemaleTanner Stage I1-86
Tanner Stage II3-169
Tanner Stage III23-351
Tanner Stage IV and V8-341
7-9 yrs1-48
10-12 yrs2-116
13-15 yrs15-333
16-17 yrs6-354
18+ yrs
Early Follicular30-250
Late Follicular200-650
Clinical SignificanceAid in evaluation of secondary amenorrhea and gonadotropin deficiency
Compliance RemarksThis test was developed and its performance characteristics determined by PAML/PSHMC Division of Laboratory Medicine. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test. However, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. PAML/PSHMC is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.
NotesUtilization of LC-MS/MS technology is suggested when increased sensitivity of estrogens is required, as is recommended for evaluating endogenous estrogen status in postmenopausal women, men, or children.

Interface Codes
typeorder codetest descriptionresult coderesult descriptionunitsdecimal placesloinc code
o82671.Z7Estrogens, Fractionated
r82671.Z7Estrogens, Fractionated82670.Z10Estradiol, Males, Children, Post-Menopg/mL 35384-7
r82671.Z7Estrogens, Fractionated82671.Z1Estronepg/mL02258-2
r82671.Z7Estrogens, Fractionated82671.Z6Estrogens, Totalpg/mL02254-1