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Test Code
SynonymsACTH Stimulation; Adrenocorticotropic Hormone Stimlutation
Container TypeSerum separator tube (gold, brick, SST, or corvac)
Supply Item Numberpicture1467
Specimen TypeFrozen serum
Preferred Volume1 mL for each timed sample
Emergency Minimum Volume0.2 mL for each timed sample
Collection ProcedureHypoadrenalism Screen: Draw cortisol immediately before and 1 hour after IV injection of 0.25 mg cosyntropin (synthetic ACTH). Procedure should be performed under physician or nurse supervision.
Specimen ProcessingSeparate serum from cells and transfer to a standard PAML aliquot tube and freeze. Clearly label specimens.
Store and TransportFrozen
StabilityRoom Temp 2 days  Refrigerated 2 weeks  Frozen (-20 °C) 3 months  
Alternate SpecimensEDTA or lithium heparin plasma
CPT Codes80400
Interface Order Code80400
Billing CodeCOR-STIM
Test ScheduleMon-Sat and STAT
Turnaround Time1-2 days
MethodElectrochemiluminescence Immunoassay
Reference Ranges
Cortisol BaselineAM Sample6.0-18.4ug/dL
PM Sample2.7-10.5
Time Drawn
Cortisol Post-StimNormal peak serum cortisol is GT 20 ug/dL, 30 to 60 minutes after 25 units cosyntropin IV.ug/dL
Time Drawn
NotesThis test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Interface Codes
typeorder codetest descriptionresult coderesult descriptionunitsdecimal placesloinc code
o80400Cortisol Stimulation Test
r80400Cortisol Stimulation Test82533Cortisolug/dL12143-6
r80400Cortisol Stimulation Test82533.Z10Cortisolug/dL121223-3
r80400Cortisol Stimulation Test82533.Z11Post Dose Time 1  19151-0
r80400Cortisol Stimulation Test82533.Z9Pre Dose Time  19151-0
r80400Cortisol Stimulation Test99199.Z76Pre Dose Time 1  29264-9
r80400Cortisol Stimulation Test99199.Z77Post Dose Time 1  49972-3