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Test Code
SynonymsACTH Stimulation; Adrenocorticotropic Hormone Stimlutation
Container TypeSerum separator tube (gold, brick, SST, or corvac)
Supply Item Numberpicture1467
Specimen TypeFrozen serum
Preferred Volume1 mL for each timed sample
Minimum Volume0.2 mL for each timed sample
Collection ProcedureHypoadrenalism Screen: Draw cortisol immediately before and 1 hour after IV injection of 0.25 mg cosyntropin (synthetic ACTH). Procedure should be performed under physician or nurse supervision.
Specimen ProcessingSeparate serum from cells within 2 hours of collection, transfer to a standard PAML aliquot tube, and freeze. Clearly label specimens.
Store and TransportFrozen
StabilityRefrigerated 10 days  
CPT Codes80400
Billing CodeCOR-STIM
Test ScheduleMon-Sat and STAT
Turnaround Time1-2 days
Test IncludesCortisol Baseline, ug/dL; Time Drawn; Cortisol Post-Stim, ug/dL; Time Drawn
Reference Ranges
Cortisol BaselineAM Sample4.3-22.4ug/dL
PM Sample3.0-16.0
Time Drawn
Cortisol Post-Stimug/dL
Normal peak serum cortisol is GT 20 ug/dL, 30 to 60 minutes after 25 units cosyntropin IV.
Time Drawn

Interface Codes
typeorder codetest descriptionresult coderesult descriptionunitsdecimal placesloinc code
o80400Cortisol Stimulation Test
r80400Cortisol Stimulation Test82533Cortisolug/dL12143-6
r80400Cortisol Stimulation Test82533.Z10Cortisolug/dL121223-3
r80400Cortisol Stimulation Test82533.Z11Post Dose Time 1  19151-0
r80400Cortisol Stimulation Test82533.Z9Pre Dose Time  19151-0
r80400Cortisol Stimulation Test99199.Z76Pre Dose Time 1  29264-9
r80400Cortisol Stimulation Test99199.Z77Post Dose Time 1  49972-3